Journal: The Journal of Biological Chemistry

Article Title: Externalized Glycolytic Enzymes Are Novel, Conserved, and Early Biomarkers of Apoptosis *

doi: 10.1074/jbc.M111.314971

Figure Lengend Snippet: Cytofluorometric analysis of externalization of other molecules. Human transformed (Jurkat) T lymphocytes that had been induced to undergo apoptosis by treatment with actinomycin D or that had been left untreated were analyzed cytofluorometrically following staining with FITC-conjugated mouse anti-human annexin II monoclonal antibody (BD Biosciences) (A) or mouse anti-calreticulin monoclonal and FITC-conjugated goat anti-rabbit IgG secondary antibodies (Enzo Life Sciences) (B). Apoptotic (solid green histograms) and viable (dashed lines) cells were identified by scatter properties and gated electronically.

Article Snippet: Human transformed (Jurkat) T lymphocytes that had been induced to undergo apoptosis by treatment with actinomycin D or that had been left untreated were analyzed cytofluorometrically following staining with FITC-conjugated mouse anti-human annexin II monoclonal antibody (BD Biosciences) ( A ) or mouse anti-calreticulin monoclonal and FITC-conjugated goat anti-rabbit IgG secondary antibodies (Enzo Life Sciences) ( B ).

Techniques: Transformation Assay, Staining

Journal: Journal of Clinical Medicine

Article Title: The Toll-like Receptor 7-Mediated Ro52 Antigen-Presenting Pathway in the Salivary Gland Epithelial Cells of Sjögren’s Syndrome

doi: 10.3390/jcm12134423

Figure Lengend Snippet: Increased expression of PLC and class I in SS patients’ LSGs. ( A ) The mean fluorescence intensity (MFI) of MHC class I (total 53 ducts from 10 SS, 19 ducts from 5 non-SS), TAP1 (53 ducts from 10 SS, 19 ducts from 5 non-SS), calreticulin (53 ducts from 10 SS, 20 ducts from 4 non-SS), ERp57 (54 ducts from 10 SS, 20 ducts from 5 non-SS) and tapasin (48 ducts from 10 SS, 28 ducts from 5 non-SS) on the ducts of labial salivary glands (LSGs) from patients with SS and non-SS subjects’ ducts. The MFI of immunostaining was captured and calculated with a hybrid cell count system. Representative samples of LSGs from SS patients ( B ) and non-SS subjects ( C ) stained with anti-class I, anti-TAP1, anti-calreticulin, anti-ERp57, anti-tapasin antibodies. mIgG1 (green), NRS (red), and NGS (red) were used as isotype controls. Hoechst was used for counterstaining the nuclei. White arrowheads: the identical ductal expression of staining for different proteins. Bar: 50 μM. Non-SS: these subjects were classified as non-SS sicca control subjects based on the AECG classification criteria. TAP1: transporter associated with antigen processing 1; ERp57: endoplasmic reticulum-resident protein 57; mIgG: mouse IgG; NRS: normal rabbit serum; NGS: normal goat serum. Significance was determined using Welch’s t -test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The primary antibodies ( ) used were rabbit anti-MHC class I polyclonal (Proteintech, Rosemont, IL, USA), mouse anti-MHC class I monoclonal (Novus Biologicals, Littleton, CO, USA), rabbit anti-Ro52 polyclonal (Cloud-Clone Corp.; Katy, TX, USA), mouse anti-Ro60 monoclonal (Santa Cruz, Dallas, TX, USA), mouse anti-ubiquitin monoclonal (Enzo Life Sciences, Farmingdale, NY, USA), rabbit anti-TAP1 monoclonal (Bioss Antibodies, Woburn, MA, USA), rabbit anti-tapasin polyclonal (GeneTex, Irvine, CA, USA), mouse anti-ERp57 monoclonal (Boster Bio, Pleasanton, CA, USA), and rabbit anti-calreticulin polyclonal antibody (LifeSpan BioSciences, Seattle, WA, USA).

Techniques: Expressing, Fluorescence, Immunostaining, Cell Counting, Staining

Journal: Journal of Clinical Medicine

Article Title: The Toll-like Receptor 7-Mediated Ro52 Antigen-Presenting Pathway in the Salivary Gland Epithelial Cells of Sjögren’s Syndrome

doi: 10.3390/jcm12134423

Figure Lengend Snippet: Increased expression of PLC and class I in SS patients’ TLR7-stimulated SGECs. Representative images in immunostaining showing the expressions of MHC class I, TAP1, calreticulin, ERp57, and tapasin in SGECs from SS patients ( n = 2) stimulated with 1 mM loxoribine for 6 h and 1000 U/mL of IFN-β for 12 h. mIgG1 (green), NRS (red), and NGS (green) were used as isotype controls. Deconvolution was performed for all images in . The insets show a magnified view of each panel. Bar: 10 μM. TAP1: transporter associated with antigen processing 1, ERp57: endoplasmic reticulum-resident protein 57, mIgG: mouse IgG, NRS: normal rabbit serum, NGS: normal goat serum.

Article Snippet: The primary antibodies ( ) used were rabbit anti-MHC class I polyclonal (Proteintech, Rosemont, IL, USA), mouse anti-MHC class I monoclonal (Novus Biologicals, Littleton, CO, USA), rabbit anti-Ro52 polyclonal (Cloud-Clone Corp.; Katy, TX, USA), mouse anti-Ro60 monoclonal (Santa Cruz, Dallas, TX, USA), mouse anti-ubiquitin monoclonal (Enzo Life Sciences, Farmingdale, NY, USA), rabbit anti-TAP1 monoclonal (Bioss Antibodies, Woburn, MA, USA), rabbit anti-tapasin polyclonal (GeneTex, Irvine, CA, USA), mouse anti-ERp57 monoclonal (Boster Bio, Pleasanton, CA, USA), and rabbit anti-calreticulin polyclonal antibody (LifeSpan BioSciences, Seattle, WA, USA).

Techniques: Expressing, Immunostaining